{"product_id":"cst-2704t","title":"CST,  2704T, Phospho-Zap-70 (Tyr493)\/Syk (Tyr526) Antibody","description":"Polyclonal Antibody for studying ZAP70 (Tyr493) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Zap-70 (Tyr493)\/Syk (Tyr526) Antibody (CST #2704) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Zap-70 (Tyr493)\/Syk (Tyr526) Antibody detects endogenous levels of Zap-70 only when phosphorylated at Tyr493. This antibody may cross-react with other activated tyrosine kinases.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr493 of human Zap-70. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe Syk family protein tyrosine kinase Zap-70 is expressed in T and NK cells and plays a critical role in mediating T cell activation in response to T cell receptor (TCR) engagement (1). Following TCR engagement, Zap-70 is rapidly phosphorylated on several tyrosine residues through autophosphorylation and transphosphorylation by the Src family tyrosine kinase Lck (2-6). Tyrosine phosphorylation correlates with increased Zap-70 kinase activity and downstream signaling events. Expression of Zap-70 is correlated with disease progression and survival in patients with chronic lymphocytic leukemia (7,8). Phosphorylation of Tyr493 within the activation loop results in enzymatic activation of Zap-70 (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; ADMIO2; FLJ17670; FLJ17679; IMD48; SRK; STCD; STD; Syk-related tyrosine kinase; Tyrosine-protein kinase ZAP-70; TZK; ZAP-70; ZAP70; zeta chain of T cell receptor associated protein kinase 70; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 70\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46797942784169,"sku":"2704T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2704t","provider":"Iright","version":"1.0","type":"link"}