{"product_id":"cst-2710s","title":"CST,  2710S, Phospho-Syk (Tyr525\/526) (C87C1) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Syk (Tyr525\/Tyr526) phosphate. Validated for WB,WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Syk (Tyr525\/526) (C87C1) Rabbit Monoclonal Antibody (CST #2710) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:100 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 16859 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Syk (Tyr525\/526) (C87C1) Rabbit Monoclonal Antibody detects endogenous levels of Syk protein only when phosphorylated at Tyr525\/526 of human Syk or Tyr519\/520 of mouse Syk. This antibody also detects Syk protein when singly phosphorylated at Tyr526 of human Syk or Tyr520 of mouse Syk. This antibody does not cross-react with other tyrosine-phosphorylated protein tyrosine kinases.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr525\/526 of human Syk.\n\u003cb\u003eBackground\u003c\/b\u003e\nSyk is a protein tyrosine kinase that plays an important role in intracellular signal transduction in hematopoietic cells (1-3). Syk interacts with immunoreceptor tyrosine-based activation motifs (ITAMs) located in the cytoplasmic domains of immune receptors (4). It couples the activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis (4). There is also evidence of a role for Syk in nonimmune cells and investigators have indicated that Syk is a potential tumor suppressor in human breast carcinomas (5). Tyr323 is a negative regulatory phosphorylation site within the SH2-kinase linker region in Syk. Phosphorylation at Tyr323 provides a direct binding site for the TKB domain of Cbl (6,7). Tyr352 of Syk is involved in the association of PLCγ1 (8). Tyr525 and Tyr526 are located in the activation loop of the Syk kinase domain; phosphorylation at Tyr525\/526 of human Syk (equivalent to Tyr519\/520 of mouse Syk) is essential for Syk function (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp313N1010; FLJ25043; FLJ37489; KSYK; p72-Syk; spleen associated tyrosine kinase; Spleen tyrosine kinase; SYK; Tyrosine-protein kinase SYK\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 72\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797943210153,"sku":"2710S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2710s","provider":"Iright","version":"1.0","type":"link"}