{"product_id":"cst-27198t","title":"CST,  27198T, beta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Beta Galactosidase. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, beta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody (CST #27198) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nbeta-Galactosidase (E2U2I) Rabbit Monoclonal Antibody recognizes endogenous levels of total β-galactosidase protein. This antibody cross-reacts with a 40 kDa protein of unknown identity in some cell extracts.\nSpecies Reactivity: Human, Mouse, Rat, Hamster, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp39 of human β-galactosidase protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nβ-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene ( ) in the presence of a β-gal substrate to identify recombinant clones (1). In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells (2), SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence (3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAcid beta-galactosidase; Beta-galactosidase; BGAL; EBP; elastin binding protein; Elastin receptor 1; elastin receptor 1, 67kDa; ELNR1; galactosidase beta 1; galactosidase, beta 1; GLB1; Lactase; MPS4B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Hm Mk\nSENSITIVITY: Endogenous\nMW (kDa): 65\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46797943767209,"sku":"27198T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27198t","provider":"Iright","version":"1.0","type":"link"}