{"product_id":"cst-27545s","title":"CST,  27545S, IL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor® 647 Conjugate)","description":"Monoclonal Antibody for studying IL6. Validated for Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, IL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 647 Conjugate) (CST #27545) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nIL-6 (E1D1N) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to full-length human IL-6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nInterleukin-6 (IL-6) is a pleiotropic cytokine with central roles in immune regulation, inflammation, hematopoiesis, and oncogenesis. Its biological activities are shared by other IL-6 family members, such as leukemia inhibitory factor and oncostatin M (1). To exert its biological effects, IL-6 must bind to the IL-6 receptor (IL-6R), composed of two alpha chains (IL-6Rα, 80 kDa) and two beta chains (IL-6Rβ or gp130, 130 kDa). Two moieties of IL-6 and two pairs of these receptor chains form a functional hexameric IL-6R complex. The subsequent intracellular signaling events are activated via gp130, which is the common signal transducer of cytokines related to IL-6. Activation of the IL-6R stimulates at least three major signaling pathways: JAK\/STAT, PI-3 kinase, and MAPK signaling pathways (2,3).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB cell stimulatory factor-2; B-cell differentiation factor; B-cell stimulatory factor 2; BSF-2; BSF2; CDF; CTL differentiation factor; HGF; HSF; Hybridoma growth factor; IFN-beta-2; IFNB2; IL-6; IL6; Interferon beta-2; interleukin 6; interleukin 6 (interferon, beta 2); interleukin BSF-2; Interleukin-6\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800369516713,"sku":"27545S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27545s","provider":"Iright","version":"1.0","type":"link"}