{"product_id":"cst-27611w2","title":"CST,  27611W2, Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Red 780 Conjugate)","description":"Monoclonal Antibody for studying JUN (Ser73) phosphate. Highly specific and rigorously validated in-house, Phospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e mFluor\u003csup\u003e™\u003c\/sup\u003e Red 780 Conjugate) (CST #27611) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-c-Jun (Ser73) (D47G9) Rabbit Monoclonal Antibody (SignalFlex™ mFluor™ Red 780 Conjugate) detects endogenous levels of c-Jun only when phosphorylated at Ser73. This antibody may also recognize JunD phosphorylated at Ser100.\nSpecies Reactivity: Human, Mouse, Rat, Monkey, Pig\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun.\n\u003cb\u003eBackground\u003c\/b\u003e\nc-Jun is a member of the Jun family containing c-Jun, JunB, and JunD, and is a component of the transcription factor activator protein-1 (AP-1). AP-1 is composed of dimers of Fos, Jun, and ATF family members and binds to and activates transcription at TRE\/AP-1 elements (reviewed in 1). Extracellular signals, including growth factors, chemokines, and stress, activate AP-1-dependent transcription. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73 through SAPK\/JNK (reviewed in 2). Knockout studies in mice have shown that c-Jun is essential for embryogenesis (3), and subsequent studies have demonstrated roles for c-Jun in various tissues and developmental processes, including axon regeneration (4), liver regeneration (5), and T cell development (6). AP-1 regulated genes exert diverse biological functions, including cell proliferation, differentiation, and apoptosis, as well as transformation, invasion and metastasis, depending on cell type and context (7-9). Other target genes regulate survival, as well as hypoxia and angiogenesis (8,10). Research studies have implicated c-Jun as a promising therapeutic target for cancer, vascular remodeling, acute inflammation, and rheumatoid arthritis (11,12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nActivator protein 1; AP-1; AP1; c-Jun; cJUN; enhancer-binding protein AP1; JUN; Jun activation domain binding protein; jun oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; p39; Proto-oncogene c-Jun; proto-oncogene cJun; Transcription factor AP-1; Transcription factor Jun; V-jun avian sarcoma virus 17 oncogene homolog; v-jun sarcoma virus 17 oncogene homolog\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk Pg\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800369746089,"sku":"27611W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27611w2","provider":"Iright","version":"1.0","type":"link"}