{"product_id":"cst-2761s","title":"CST,  2761S, Numb (C44B4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Numb. Validated for WB,IP,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Numb (C44B4) Rabbit Monoclonal Antibody (CST #2761) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNumb (C44B4) Rabbit Monoclonal Antibody detects endogenous levels of Numb protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNumb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nc14_5527; C14orf41; h-Numb; NUMB; NUMB endocytic adaptor protein; numb homolog; numb homolog (Drosophila); Protein numb homolog; Protein S171; S171\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 72, 74\nSource\/Isotype: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798014841001,"sku":"2761S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2761s","provider":"Iright","version":"1.0","type":"link"}