{"product_id":"cst-27782s","title":"CST,  27782S, CDK4 (D9G3E) Rabbit Monoclonal Antibody (Biotinylated)","description":"Monoclonal Antibody for studying Cdk4. Validated for Western Blotting. Highly specific and rigorously validated in-house, CDK4 (D9G3E) Rabbit Monoclonal Antibody (Biotinylated) (CST #27782) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 140 mM NaCl, 3 mM KCI, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCDK4 (D9G3E) Rabbit Monoclonal Antibody (Biotinylated) recognizes endogenous levels of total CDK4 protein. In COS-7 cells, this antibody recognizes a 40 kDa background band of unknown origin. This antibody does not cross-react with CDK1, 2, or 6.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CDK4 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclin-dependent kinase activity is regulated by T-loop phosphorylation (Thr172 in the case of CDK4), by the abundance of their cyclin partners, and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins (1). The inactive ternary complex of CDK4\/cyclin D and p27 Kip1\/Cip1 requires extracellular mitogenic stimuli for the release and degradation of p27, which affects progression through the restriction point and pRb-dependent entry into S-phase (2). The active complex of CDK4\/cyclin D targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression (3). In HeLa cells, upon UV irradiation, upregulation of p16 INK4A association with CDK4\/cyclin D3 leads to a G2 delay, implicating CDK4\/cyclin D3 activity in progression through the G2-phase of the cell cycle (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDK4; cell division kinase 4; Cell division protein kinase 4; CMM3; cyclin dependent kinase 4; Cyclin-dependent kinase 4; melanoma cutaneous malignant, 3; MGC14458; PSK-J3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 30\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800370237609,"sku":"27782S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27782s","provider":"Iright","version":"1.0","type":"link"}