{"product_id":"cst-27960t","title":"CST,  27960T, LIMP-2\/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SCARB2 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, LIMP-2\/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody (CST #27960) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:200 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:50 - 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . For a carrier free (BSA and azide free) version of this product see product # 69463 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nLIMP-2\/SCARB2 (E2Z5F) Rabbit Monoclonal Antibody recognizes endogenous levels of total LIMP-2\/SCARB2 protein. LIMP-2\/SCARB2 is glycosylated at multiple sites, and it migrates at 80 kDa and 54 kDa in its glycosylated and unglycosylated states, respectively.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly467 of mouse LIMP-2\/SCARB2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nLysosomal integral membrane protein type 2 (LIMP-2, also known as SCARB2) is a lysosomal membrane glucoprotein encoded by the gene. As a lysosome-enriched protein, LIMP-2\/SCARB2 contributes to endosomal and lysosomal transport and function in the cell. β-glucocerebrosidase (GBA), a lysosomal enzyme defective in Gaucher disease and genetically linked to Parkinson's disease, is targeted to lysosomes by binding directly to LIMP-2\/SCARB2 via a mannose 6-phosphate-independent pathway (1-3). LIMP-2\/SCARB2 may also play a role in the transport of cholesterol from the lysosome to the membrane, an important function as cholesterol contributes to various biophysical properties of cell membranes and may contribute to various diseases (4,5). Interestingly, SCARB2 is upregulated in neurodegenerative diseases, including Lewy body disease (6), and genetic variants in the gene encoding SCARB2 are linked to Parkinson's disease (7), suggesting that altered lysosomal function may contribute to neuropathology in these diseases. LIMP-2\/SCARB2 also serves as a receptor for enterovirus 71 and coxsackieviruses (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n85 kDa lysosomal membrane sialoglycoprotein; 9330185J12Rik; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 2; Cd36l; Cd36l2; LGP; LGP85; LIM; LIMP II; LIMP-2; Lysosome membrane protein 2; Lysosome membrane protein II; MLGP85; Scarb2; Scavenger receptor class B member 2; scavenger receptor class B, member 2; SCRB2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 54, 80\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798017659049,"sku":"27960T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27960t","provider":"Iright","version":"1.0","type":"link"}