{"product_id":"cst-27987s","title":"CST,  27987S, Adora2b\/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying ADORA2B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Adora2b\/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody (CST #27987) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAdora2b\/Adenosine Receptor A2b (E8V7W) Rabbit Monoclonal Antibody recognizes endogenous levels of total mouse Adora2b\/Adenosine Receptor A2b protein. This antibody cross-reacts with human Adenosine receptor A2a protein but not with mouse Adenosine receptor A2a protein or human Adenosine receptor A2b protein. This antibody detects a 140 kDa band of unknown origin, and in human cell lines, detects a 42 kDa band of unknown origin.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse Adora2b\/Adenosine Receptor A2b protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAdenosine receptor A2b (A2B adenosine receptor, A2BAR, ADORA2B) belongs to the adenosine receptor group of G protein-coupled receptors (GPCRs) (1-6). The adenosine receptors are divided into A1, A2, and A3 subtypes based on their ability to inhibit or stimulate adenyl cyclase (1-3). Within the A2 subtype, the high-affinity receptor is designated the A2A adenosine receptor, and the low-affinity receptor is the A2B adenosine receptor (1-3). Adenosine receptor A2b has been identified as a regulator of inflammation and vascular adhesion, primarily via signals from hematopoietic cells to the vasculature (3). Adenosine receptor A2b couples to G proteins to activate adenyl cyclase, leading to increased intracellular cAMP levels, as well as activation of phospholipase C (PLC), leading to increased intracellular calcium (4-6). Multiple mediators associated with inflammation, such as LPS, TNF-α, and IFN-γ, have been demonstrated to increase A2BAR expression in certain cell types (4-6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAA2BR; adenosine A2b receptor; Adenosine receptor A2b; ADORA2; ADORA2B\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 30-50\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800086270121,"sku":"27987S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-27987s","provider":"Iright","version":"1.0","type":"link"}