{"product_id":"cst-28034t","title":"CST,  28034T, CD27 (E6S8H) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD27. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, CD27 (E6S8H) Rabbit Monoclonal Antibody (CST #28034) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD27 (E6S8H) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD27 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu249 of human CD27 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD27 (TNFRSF7) is a transmembrane protein of the TNF receptor superfamily (TNFRSF). It is mainly expressed on lymphoid cells (also on early hematopoietic precursor cells in mice) (1,2). CD27 is considered a phenotypic marker for memory B cells and is also used to identify B regulatory (Breg) cells (3,4). It is constitutively expressed on naïve CD4 and CD8 T cells, and its expression is further upregulated upon T cell activation. CD27 is one of the two most important co-stimulatory receptors for T cell priming (the other one is CD28). While CD28 co-stimulatory signal mainly triggers cell proliferation, CD27 co-stimulatory signal primarily promotes cell survival and differentiation (5,6). Upon binding to its ligand CD70, CD27 activates the NF-κB and JNK signaling pathways through TNFR-associated factors (TRAFs), the adaptor molecules that are associated with CD27 cytoplasmic tail domain. Upon activation, CD27 is shed from the cell surface, and soluble CD27 is used as a marker of T cell activation (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD27; CD27 antigen; CD27 molecule; CD27L receptor; MGC20393; S152; S152. LPFS2; T cell activation antigen CD27; T cell activation antigen S152; T cell antivation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; Tp55; tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 55\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798018150569,"sku":"28034T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28034t","provider":"Iright","version":"1.0","type":"link"}