{"product_id":"cst-28147t","title":"CST,  28147T, Nectin-1\/PVRL1 (F7J9O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying nectin 1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Nectin-1\/PVRL1 (F7J9O) Rabbit Monoclonal Antibody (CST #28147) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNectin-1\/PVRL1 (F7J9O) Rabbit Monoclonal Antibody recognizes endogenous levels of total nectin-1\/PVRL1 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln478 of human nectin-1\/PVRL1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNectin cell adhesion molecule 1 (Nectin-1), also known as poliovirus receptor-related 1 (PVRL1) and CD111, is a single-pass type I membrane glycoprotein and is part of the immunoglobulin (Ig) superfamily of cell adhesion proteins (1). Nectins are composed of an ectodomain containing three tandem Ig-like regions arranged as an N-terminal variable-like (V) domain followed by two constant-like (C) domains, a single transmembrane region, and a cytoplasmic domain that binds to the actin cytoskeleton through the adaptor protein afadin (2). Nectin-1 plays important roles in adherens junction (AJ) formation in epithelial cells and neuronal synapses (1,3). Like other nectin family members, nectin-1 is used as an entry receptor for several mammalian viruses, including herpes simplex virus types 1 and 2 (HSV-1 and HSV-2), porcine pseudorabies virus (PRV), bovine herpes virus type 1 (BHV-1), and simian B virus (4-6). Nectin-1 also plays a role in the immune system, where it interacts with CD96 expressed on natural killer (NK) cells and T cell subsets, and modulates cancer cell and virally infected cell clearance (7,8).  Â\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD111; CLPED1; ectodermal dysplasia 4 (Margarita Island type); ED4; herpes simplex virus type 1 sensitivity; Herpes virus entry mediator C; Herpesvirus entry mediator C; Herpesvirus Ig-like receptor; HIgR; HV1S; HVEC; MGC142031; MGC16207; NECT1; nectin 1; Nectin cell adhesion molecule 1; Nectin-1; NECTIN1; OFC7; poliovirus receptor-like 1; poliovirus receptor-related 1 (herpesvirus entry mediator C); Poliovirus receptor-related protein 1; PRR; PRR1; PVRL1; PVRR; PVRR1; SK-12\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 100\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798052393129,"sku":"28147T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28147t","provider":"Iright","version":"1.0","type":"link"}