{"product_id":"cst-28182t","title":"CST,  28182T, CD72 (E4K4A) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying CD72. Validated for Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, CD72 (E4K4A) Rabbit Monoclonal Antibody (CST #28182) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Live): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCD72 (E4K4A) Rabbit Monoclonal Antibody recognizes endogenous levels of total CD72 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln314 of human CD72 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCD72 is an inhibitory co-receptor of the B cell antigen receptor (BCR) and is expressed primarily on B cells (1,2). The cytoplasmic domain of CD72 contains immunoreceptor tyrosine-based inhibition motifs (ITIMs) that are phosphorylated by Lyn when Lyn associates with BCR (3-5). This phosphorylation leads to recruitment of SHP-1 to CD72 and negative regulation of BCR signaling (4,5). The extracellular portion of CD72 contains a C-type lectin domain, and binding its ligand, CD100\/Semaphorin-4D, leads to dissociation of CD72 and SHP-1 and thus relief of BCR signaling suppression (6,7). Recently, Semaphorin-3A was also reported to act as a ligand for CD72; however, unlike CD100\/Semaphorin-4D, association with Semaphorin-3A induces CD72 ITAM phosphorylation and association of CD72 with SHP-1 (8). CD72 also plays a role in systemic lupus erythematosus (SLE); through its recognition of the self-antigen Sm\/RNP, CD72 suppresses the TLR7-dependent B cell response to nucleic acid self-antigens, inhibiting SLE development (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nB-cell differentiation antigen CD72; CD72; CD72 antigen; CD72 molecule; CD72b; Lyb-2; LYB2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800330752169,"sku":"28182T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28182t","provider":"Iright","version":"1.0","type":"link"}