{"product_id":"cst-2821s","title":"CST,  2821S, Phospho-PLC gamma1 (Tyr783) Antibody","description":"Polyclonal Antibody for studying PLCg1 (Tyr783) phosphate. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-PLC gamma1 (Tyr783) Antibody (CST #2821) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PLC gamma1 (Tyr783) Antibody detects PLCγ1 only when phosphorylated at tyrosine 783. This antibody does not cross-react with phosphorylated PLCγ2 or other PLCs.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding tyrosine 783 of human PLCγ1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nPhosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1248 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n1-phosphatidyl-D-myo-inositol-4,5-bisphosphate; 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma 1; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1; inositoltrisphosphohydrolase; monophosphatidylinositol phosphodiesterase; NCKAP3; phosphatidylinositol phospholipase C; phosphoinositidase C; phosphoinositide phospholipase C; Phosphoinositide phospholipase C-gamma-1; phospholipase C gamma 1; phospholipase C-148; Phospholipase C-gamma-1; Phospholipase C-II; phospholipase C, gamma 1; phospholipase C, gamma 1 (formerly subtype 148); PLC-148; PLC-gamma-1; PLC-II; PLC1; PLC148; PLCG1; PLCgamma1; triphosphoinositide phosphodiesterase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 155\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798052786345,"sku":"2821S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2821s","provider":"Iright","version":"1.0","type":"link"}