{"product_id":"cst-28305sf","title":"CST,  28305SF, S100A9 (E6P3Q) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying S100A9. Validated for ELISA+. Highly specific and rigorously validated in-house, S100A9 (E6P3Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #28305) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free.\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nS100A9 (E6P3Q) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total S100A9 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a fragment of human S100A9 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nS100A8 and S100A9 are calcium-binding proteins that form a noncovalent heterodimer present in monocytes, neutrophils, macrophages, and some epithelial cells (1,2). S100A8 and S100A9 are secreted by a tubulin-dependent mechanism during inflammatory conditions and have antimicrobial and chemotactic functions (3-5). Extracellular S100A8\/S100A9 also induces an inflammatory response in endothelial cells, including induction of proinflammatory chemokines and adhesion molecules and increased vascular permeability (6). S100A8\/S100A9 induces and recruits myeloid-derived suppressor cells (MDSC) in tumor-bearing mice (7). MDSC produce additional S100A8\/S100A9 themselves, resulting in a positive feedback mechanism that sustains MDSC accumulation (7). S100A8\/S100A9 is also highly expressed in psoriatic skin, where it directly upregulates transcription of complement protein C3, which contributes to disease (8). In addition, tumor-infiltrating myeloid cells induce expression of S100A8 and S100A9 in cancer cells, which increases invasiveness and metastasis (9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n60B8AG; CAGB; calgranulin B; Calgranulin-B; Calprotectin L1H subunit; CFAG; CGLB; L1AG; Leukocyte L1 complex heavy chain; LIAG; MAC387; MIF; Migration inhibitory factor-related protein 14; MRP-14; MRP14; NIF; p14; Protein S100-A9; S100 calcium binding protein A9; S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A9; S10A9\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800307388585,"sku":"28305SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28305sf","provider":"Iright","version":"1.0","type":"link"}