{"product_id":"cst-28386s","title":"CST,  28386S, PTPRJ\/DEP-1 (F4G8K) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PTPRJ. Validated for WB,IP,F,F. Available in 2 sizes. Highly specific and rigorously validated in-house, PTPRJ\/DEP-1 (F4G8K) Rabbit Monoclonal Antibody (CST #28386) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\nFlow Cytometry (Live): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Flow Cytometry (Fixed\/Permeabilized), Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPTPRJ\/DEP-1 (F4G8K) Rabbit Monoclonal Antibody recognizes endogenous levels of total PTPRJ\/DEP-1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to a fragment of the extracellular domain of human PTPRJ\/DEP-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein-tyrosine phosphatase receptor type J (PTPRJ), also known as density-enhanced phosphatase-1 (DEP-1), is a heavily glycosylated, type 1 transmembrane protein. The extracellular region of PTPRJ\/DEP-1 contains multiple copies of the fibronectin type III motif, while its intracellular region harbors a single PTP catalytic domain (1). PTPRJ\/DEP-1 is expressed at varying levels on multiple hematopoietic cell lineages and has been shown to modulate signaling downstream of the TCR complex (2,3). Research studies have suggested that PTPRJ\/DEP-1 also plays a role in non-hematopoietic cell biology through its ability to inhibit cell growth in response to increasing cell density (4). Indeed, PTPRJ\/DEP-1-dependent dephosphorylation of mitogenic molecules, such as c-Met and PDGFR-β, has been shown to attenuate signaling downstream of these RTKs (5,6). In many contexts, PTPRJ\/DEP-1 functions as a tumor suppressor, and its expression is lost in multiple types of human cancers (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD148; CD148 antigen; Density-enhanced phosphatase 1; DEP-1; DEP1; HPTP eta; HPTPeta; human density enhanced phosphatase-1; protein tyrosine phosphatase receptor type J; protein tyrosine phosphatase, receptor type, J; protein tyrosine phosphatase, receptor type, J polypeptide; Protein-tyrosine phosphatase eta; Protein-tyrosine phosphatase receptor type J; PTPRJ; R-PTP-eta; R-PTP-J; Receptor-type tyrosine-protein phosphatase eta; SCC1; susceptibility to colon cancer 1, mouse, homolog of\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 240\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798053572777,"sku":"28386S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28386s","provider":"Iright","version":"1.0","type":"link"}