{"product_id":"cst-2841s","title":"CST,  2841S, Phospho-C\/EBP alpha (Ser21) Antibody","description":"Polyclonal Antibody for studying C\/EBP-alpha (Ser21) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-C\/EBP alpha (Ser21) Antibody (CST #2841) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-C\/EBP alpha (Ser21) Antibody detects endogenous levels of C\/EBPα only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C\/EBP isoforms.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human C\/EBPα. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCCAAT\/enhancer-binding proteins (C\/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function, and inflammatory response (1). Six members of the family have been characterized (C\/EBPα, β, δ, γ, ε, and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C\/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-I stimulate the dephosphorylation of C\/EBPα, which may play a key role in insulin-induced repression of GLUT4 transcription (3). Phosphorylation of C\/EBPα at Thr222, Thr226, and Ser230 by GSK-3 seems to be required for adipogenesis (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nC\/EBP alpha; C\/EBP-alpha; CCAAT enhancer binding protein alpha; CCAAT\/enhancer binding protein (C\/EBP), alpha; CCAAT\/enhancer-binding protein alpha; CEBP; CEBPA\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798054031529,"sku":"2841S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2841s","provider":"Iright","version":"1.0","type":"link"}