{"product_id":"cst-2851s","title":"CST,  2851S, Phospho-(Ser\/Thr) ATM\/ATR Substrate Antibody","description":"Polyclonal Antibody for studying . Validated for Western Blotting,Immunoprecipitation,Peptide ELISA (DELFIA). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-(Ser\/Thr) ATM\/ATR Substrate Antibody (CST #2851) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:20\nPeptide ELISA (DELFIA): 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-(Ser\/Thr) ATM\/ATR Substrate Antibody detects endogenous levels of proteins containing the ATM\/ATR substrate motif. This antibody preferentially binds peptides and proteins that contain phospho-Ser\/Thr preceded by Leu or similar hydrophobic amino acids at the -1 position and followed by Gln at the +1 position. This antibody does not cross-react with corresponding nonphosphorylated sequences or with other phospho-Ser\/Thr-containing motifs.\nSpecies Reactivity: All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with synthetic phospho-(Ser\/Thr) ATM\/ATR substrate peptides . Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nAtaxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are related kinases that regulate cell cycle checkpoints and DNA repair (1). The identified substrates for ATM are p53, p95\/NBS1, MDM2, Chk2, BRCA1, CtIP, 4E-BP1, and Chk1 (1,2) The essential requirement for the substrates of ATM\/ATR is S\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: All\nSENSITIVITY: Endogenous\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800209051817,"sku":"2851S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2851s","provider":"Iright","version":"1.0","type":"link"}