{"product_id":"cst-2857s","title":"CST,  2857S, Phospho-DUSP1\/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying DUSP1 (Ser359) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-DUSP1\/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody (CST #2857) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-DUSP1\/MKP1 (Ser359) (125E2) Rabbit Monoclonal Antibody detects endogenous levels of DUSP1 only when phosphorylated at serine 359.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser359 of human DUSP1.\n\u003cb\u003eBackground\u003c\/b\u003e\nMAP kinases are inactivated by a family of dual-specificity protein phosphatases (DUSP) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. DUSP1\/MKP1 (MAP Kinase phosphatase 1) is primarily localized in the nucleus, and has broad substrate specificity towards p44\/42 MAPK, p38 MAPK and SAPK\/JNK (1-3). DUSP1's transcription and activity are tightly regulated. First, DUSP1 is transcriptionally induced through p44\/42 MAPK, p53 and Jak2 (2,4,5). Second, DUSP1 is phosphorylated by p44\/42 MAPK at Ser359 and Ser364 in its carboxy-terminal region, which inhibits DUSP1 degradation through the ubiquitin pathway (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCL 100; CL100; dual specificity phosphatase 1; Dual specificity protein phosphatase 1; Dual specificity protein phosphatase hVH1; DUS1; DUSP1; HVH1; MAP kinase phosphatase 1; Mitogen-activated protein kinase phosphatase 1; MKP-1; MKP1; Protein-tyrosine phosphatase CL100; PTPN10; serine\/threonine specific protein phosphatase; VH1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798055243945,"sku":"2857S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2857s","provider":"Iright","version":"1.0","type":"link"}