{"product_id":"cst-28580s","title":"CST,  28580S, SynGAP-beta (F9H8E) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SynGAP iso2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SynGAP-beta (F9H8E) Rabbit Monoclonal Antibody (CST #28580) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSynGAP-beta (F9H8E) Rabbit Monoclonal Antibody recognizes endogenous levels of total SynGAP-β protein. This antibody does not cross-react with other SynGAP isoforms.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SynGAP-β protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nSynGAP is a synaptic GTPase-activating protein selectively expressed in the brain and found at higher concentrations, specifically at excitatory synapses in the mammalian forebrain. SynGAP has a PH domain, a C2 domain, and a highly conserved RasGAP domain, which negatively regulates both Ras activity and its downstream signaling pathways. SynGAP interacts with the PDZ domains of SAP102, as well as PSD95, a postsynaptic scaffolding protein that couples SynGAP to NMDA receptors (1). SynGAP is phosphorylated by Ca \/calmodulin-dependent protein kinase II (CaMKII) at Ser765 and Ser1123, among other sites (2,3). Phosphorylation of SynGAP results in stimulation of the GTPase activity of Ras, and PSD95-dependent CaMKII phosphorylation of SynGAP increases after transient brain ischemia (1,4). SynGAP is implicated in NMDAR- and CaMKII-dependent regulation of AMPAR trafficking and plays an important role in synaptic plasticity (3,5). SynGAP is critical during neuronal development as mice lacking SynGAP protein die postnatally. Furthermore, SynGAP mutant mice have reduced long-term potentiation (LTP) and perform poorly in spatial memory tasks (6). Alternative splicing of the gene generates four isoforms with distinct C-terminal sequences (α1, α2, β, and γ). These isoforms exhibit unique spatiotemporal expression patterns, subcellular localizations, and proposed functions in health and disease (7-9).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp761G1421; KIAA1938; MRD5; Neuronal RasGAP; Ras GTPase-activating protein SynGAP; Ras\/Rap GTPase-activating protein SynGAP; RASA1; RASA5; SYGP1; synaptic Ras GTPase activating protein 1; synaptic Ras GTPase activating protein 1 homolog; synaptic Ras GTPase activating protein 1 homolog (rat); synaptic Ras GTPase activating protein, 135kDa; Synaptic Ras GTPase-activating protein 1; Synaptic Ras-GAP 1; SYNGAP; SynGAP iso2; SYNGAP1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 140\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798055342249,"sku":"28580S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28580s","provider":"Iright","version":"1.0","type":"link"}