{"product_id":"cst-28585w2","title":"CST,  28585W2, HPK1 (E1C3L) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 555 Conjugate)","description":"Monoclonal Antibody for studying HPK1. Highly specific and rigorously validated in-house, HPK1 (E1C3L) Rabbit Monoclonal Antibody (SignalFlex\u003csup\u003e™\u003c\/sup\u003e Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 555 Conjugate) (CST #28585) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions\/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nHPK1 (E1C3L) Rabbit mAb (SignalFlex™ Alexa Fluor\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HPK1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nHematopoietic progenitor kinase 1 (HPK1) is a member of the germinal center kinases that is predominantly expressed in hematopoietic cells. HPK1 is a key component linking T or B cell receptors to SAPK\/JNK and IkappaB kinase pathways in lymphocytes (1-3). Through its proline-rich motif, HPK1 associates with multiple SH3 domain-containing adaptor proteins, including GRB2, Nck, Crk, SLP-76, and BLNK. Phosphorylation of Tyr379 by Syk is necessary for HPK1 association with BLNK and SLP-76, as well as for HPK1 activity (4,5). Caspase cleavage of HPK1 also modulates the biological function of HPK1 (3,6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHematopoietic progenitor kinase; hematopoietic progenitor kinase 1; HPK1; M4K1; MAP4K1; MAPK\/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; Mitogen-activated protein kinase kinase kinase kinase 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800372105385,"sku":"28585W2","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28585w2","provider":"Iright","version":"1.0","type":"link"}