{"product_id":"cst-28711s","title":"CST,  28711S, Aurora B\/AIM1 (E3R8N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Aurora B. Validated for WB,WB,IP,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Aurora B\/AIM1 (E3R8N) Rabbit Monoclonal Antibody (CST #28711) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:100\nImmunofluorescence (Immunocytochemistry): 1:800 - 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nAurora B\/AIM1 (E3R8N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Aurora B\/AIM1 protein.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Auroro B\/AIM1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAurora A (AIK) is a cell cycle-regulated Ser\/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment\/segregation, and altered survivin localization (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIK2; AIM-1; AIM1; AIRK2; ARK-2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; Aurora 1; Aurora B; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; Aurora-related kinase 2; Aurora\/IPL1-related kinase 2; IPL1; PPP1R48; protein phosphatase 1, regulatory subunit 48; serine\/threonine kinase 12; Serine\/threonine-protein kinase 12; Serine\/threonine-protein kinase 5; Serine\/threonine-protein kinase aurora-B; STK-1; STK1; STK12; STK5\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 40\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798056423593,"sku":"28711S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-28711s","provider":"Iright","version":"1.0","type":"link"}