{"product_id":"cst-2890t","title":"CST,  2890T, Oct-4A (C52G3) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Oct4A. Validated for WB,IHC,IF,F,ChIP,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Oct-4A (C52G3) Rabbit Monoclonal Antibody (CST #2890) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP and ChIP-seq results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:600 - 1:2400\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\nChromatin IP: 1:50\nChromatin IP-seq: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85676 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP, Chromatin IP-seq\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nOct-4A (C52G3) Rabbit Monoclonal Antibody detects endogenous levels of total Oct-4A protein. Based on high sequence homology, this antibody may also detect OCT4-PG1.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A.\n\u003cb\u003eBackground\u003c\/b\u003e\nOct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nMGC22487; Oct-3; Oct-4; OCT3; OCT3A; OCT4; OCT4A; Octamer-binding protein 3; Octamer-binding protein 4; Octamer-binding transcription factor 3; octamer-binding transcription factor-3; OTF-3; OTF3; OTF4; PO5F1; POU class 5 homeobox 1; POU class 5 homeobox 1 transcript variant OCT4B1; POU class 5 homeobox 1 transcript variant OCT4B2; POU class 5 homeobox 1 transcript variant OCT4B3; POU class 5 homeobox 1 transcript variant OCT4B4; POU class 5 homeobox 1 transcript variant OCT4B5; POU class 5 homeobox 1 transcript variant OCT4B6; POU classV homeobox 1 variant 2; POU domain transcription factor OCT4; POU domain, class 5, transcription factor 1; POU-type homeodomain-containing DNA-binding protein; POU5F1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798057570473,"sku":"2890T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2890t","provider":"Iright","version":"1.0","type":"link"}