{"product_id":"cst-2895l","title":"CST,  2895L, CHOP (L63F7) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying CHOP. Validated for WB,IP,IF,F,ChIP. Available in 3 sizes. Highly specific and rigorously validated in-house, CHOP (L63F7) Mouse Monoclonal Antibody (CST #2895) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFor optimal ChIP results, use 2.5 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits.\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Immunocytochemistry): 1:400 - 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:250 - 1:1000\nChromatin IP: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 78063 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized), Chromatin IP\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCHOP (L63F7) Mouse Monoclonal Antibody detects endogenous levels of total CHOP protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CHOP protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCHOP was identified as a C\/EBP-homologous protein that inhibits C\/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAltDDIT3; alternative DDIT3 protein; C\/EBP homologous protein; C\/EBP zeta; C\/EBP-homologous protein; C\/EBP-homologous protein 10; C\/EBPzeta; CCAAT\/enhancer-binding protein homologous protein; CEBPZ; CHOP; CHOP-10; CHOP10; DDIT-3; DDIT3; DNA damage inducible transcript 3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth arrest and DNA damage-inducible protein GADD153; growth arrest- and DNA damage-inducible; MGC4154\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 27\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46798057996457,"sku":"2895L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2895l","provider":"Iright","version":"1.0","type":"link"}