{"product_id":"cst-2904s","title":"CST,  2904S, VEGF Receptor 2 Control Proteins","description":"Protein Control Kit for studying VEGFR2 in the research area.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nBoil for 3 minutes prior to use. Load 15 µl (20 ng) of phosphorylated and nonphosphorylated VEGF Receptor 2 Control Proteins per lane.\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w\/v SDS, 10% glycerol, 50 mM DTT, 0.01% w\/v phenol red or bromophenol blue. Store at -20°C or at -80°C for long term storage.\n\u003cb\u003eBackground\u003c\/b\u003e\nVascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Major autophosphorylation sites of VEGFR2 are located in the kinase insert domain (Tyr951\/996) and in the tyrosine kinase catalytic domain (Tyr1054\/1059) (2). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (3). Phosphorylation at Tyr1212 provides a docking site for GRB2 binding and phospho-Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ, as well as Shb (1,4,5). Signaling from VEGFR2 is necessary for the execution of VEGF-stimulated proliferation, chemotaxis and sprouting, as well as survival of cultured endothelial cells and angiogenesis (6-8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nextracts; Flk; KDR; lysates; vegfr2","brand":"CST","offers":[{"title":"Default Title","offer_id":46800722264233,"sku":"2904S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2904s","provider":"Iright","version":"1.0","type":"link"}