{"product_id":"cst-29362s","title":"CST,  29362S, Phospho-Beclin-1 (Ser90) (E3X1I) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Beclin 1 (Ser90) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Beclin-1 (Ser90) (E3X1I) Rabbit Monoclonal Antibody (CST #29362) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Beclin-1 (Ser90) (E3X1I) Rabbit Monoclonal Antibody recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser90. This antibody may not recognize Beclin-1 when dually phosphorylated at Ser90 and Ser93.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser90 of human Beclin-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nAutophagy is a catabolic process for the autophagosomic-lysosomal degradation of proteins activated in response to nutrient deprivation and in neurodegenerative conditions (1). One of the proteins critical to this process is Beclin-1, the mammalian orthologue of the yeast autophagy protein Apg6\/Vps30 (2). Beclin-1 can complement defects in yeast autophagy caused by loss of Apg6 and can also stimulate autophagy when overexpressed in mammalian cells (3). Mammalian Beclin-1 was originally isolated in a yeast two-hybrid screen for Bcl-2 interacting proteins and has been shown to interact with Bcl-2 and Bcl-xL, but not with Bax or Bak (4). While Beclin-1 is generally ubiquitously expressed, research studies have shown it is monoallelically deleted in 40-75% of sporadic human breast and ovarian cancers (5). Beclin-1 is localized within cytoplasmic structures including the mitochondria, although overexpression of Beclin-1 reveals some nuclear staining and CRM1-dependent nuclear export (6). Investigators have demonstrated that Beclin-1 mice die early in embryogenesis and Beclin-1 mice have a high incidence of spontaneous tumors. Stem cells from the mice demonstrate an altered autophagic response, although responses to apoptosis appeared normal (7). Researchers have also found that overexpression of Beclin-1 in virally infected neurons resulted in significant protection against Sindbis virus-induced disease and neuronal apoptosis (4). Several studies have indicated that autophagy is enhanced by phosphorylation of Beclin-1 at Ser90 triggered by stress-responsive kinases including MAPKAPK-2\/MAPKAPK-3, DAPK3, and CaMKII (8-10).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nATG6; ATG6 autophagy related 6 homolog; beclin 1; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); beclin 1, autophagy related; Beclin-1; Beclin-1-C 35 kDa; Beclin-1-C 37 kDa; beclin1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; testis secretory sperm-binding protein Li 215e; VPS30\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 60\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798060257449,"sku":"29362S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-29362s","provider":"Iright","version":"1.0","type":"link"}