{"product_id":"cst-2936t","title":"CST,  2936T, Cyclin D3 (DCS22) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Cyclin D3. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin D3 (DCS22) Mouse Monoclonal Antibody (CST #2936) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:2000\nImmunohistochemistry (Paraffin): 1:800 - 1:3200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 99580 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCyclin D3 (DCS22) Mouse Monoclonal Antibody detects endogenous levels of total cyclin D3 protein. This antibody does not cross-react with cyclin D1 or cyclin D2.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant human cyclin D3 corresponding to residues 241-260.\n\u003cb\u003eBackground\u003c\/b\u003e\nActivity of the cyclin-dependent kinases CDK4 and CDK6 is regulated by T-loop phosphorylation, by the abundance of their cyclin partners (the D-type cyclins), and by association with CDK inhibitors of the Cip\/Kip or INK family of proteins (1). The inactive ternary complex of cyclin D\/CDK4 and p27 Kip1 requires extracellular mitogenic stimuli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phase (2). The active complex of cyclin D\/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1\/S-phase gene expression (3). Levels of cyclin D protein drop upon withdrawal of growth factors through downregulation of protein expression and phosphorylation-dependent degradation (4). Although the D-type cyclins are not fully redundant, cyclin D3, like D1, plays a prominent role in differentiation and proliferation, which correlates with higher expression levels of cyclin D3 in various cancers (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCCND3; cyclin D3; D3-type cyclin; G1\/S-specific cyclin D3; G1\/S-specific cyclin-D3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 31\nSource\/Isotype: Mouse IgG1","brand":"CST","offers":[{"title":"Default Title","offer_id":46798060191913,"sku":"2936T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2936t","provider":"Iright","version":"1.0","type":"link"}