{"product_id":"cst-2946s","title":"CST,  2946S, p21 Waf1\/Cip1 (DCS60) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying p21 Waf1\/Cip1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 3 sizes. Highly specific and rigorously validated in-house, p21 Waf1\/Cip1 (DCS60) Mouse Monoclonal Antibody (CST #2946) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:2000\nImmunohistochemistry (Paraffin): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np21 Waf1\/Cip1 (DCS60) Mouse Monoclonal Antibody detects endogenous levels of total p21 protein. This antibody does not cross-react with other cdk inhibitors.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant human p21 corresponding to the amino-terminal portion of p21.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe tumor suppressor protein p21 Waf1\/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1\/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2\/cyclin B or CDK2\/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAP20; CDK-interacting protein 1; CDK-interaction protein 1; CDKN1; CDKN1A; CDN1A; CIP1; cyclin dependent kinase inhibitor 1A; Cyclin-dependent kinase inhibitor 1; cyclin-dependent kinase inhibitor 1A (p21, Cip1); DNA synthesis inhibitor; MDA-6; MDA6; melanoma differentiation associated protein 6; Melanoma differentiation-associated protein 6; p21; p21CIP1; p21Cip1\/Waf1; PIC1; SDI1; WAF1; wild-type p53-activated fragment 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 21\nSource\/Isotype: Mouse IgG2a","brand":"CST","offers":[{"title":"Default Title","offer_id":46798060781737,"sku":"2946S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2946s","provider":"Iright","version":"1.0","type":"link"}