{"product_id":"cst-2954t","title":"CST,  2954T, Smac\/Diablo (79-1-83) Mouse Monoclonal Antibody","description":"Monoclonal Antibody for studying Diablo\/Smac. Validated for Western Blotting,Immunoprecipitation,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Smac\/Diablo (79-1-83) Mouse Monoclonal Antibody (CST #2954) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunohistochemistry (Paraffin): 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 19293 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSmac\/Diablo (79-1-83) Mouse Monoclonal Antibody detects endogenous levels of processed and unprocessed human Smac\/Diablo. The antibody reacts weakly with mouse and rat Smac\/Diablo.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a full length peptide corresponding to human Smac\/Diablo raised in E-coli.\n\u003cb\u003eBackground\u003c\/b\u003e\nSmac\/Diablo is a 21 kDa mammalian mitochondrial protein that functions as a regulatory component during apoptosis (1,2). Upon mitochondrial stress, Smac\/Diablo is released from mitochondria and competes with caspases for binding of IAPs (inhibitor of apoptosis proteins) (1,2). The interaction of Smac\/Diablo with IAPs relieves the inhibitory effect of the IAPs on caspases (3,4). This interaction involves mainly the amino-terminal residues of Smac\/Diablo with the BIR3 region of XIAP, supplemented with several other hydrophobic interactions between the helical structures of Smac\/Diablo and other areas of BIR3 (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n0610041G12Rik; DBLOH; DFNA64; DIABLO; diablo homolog (Drosophila); Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial Smac protein; Second mitochondria-derived activator of caspase; SMAC; SMAC3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 21\nSource\/Isotype: Mouse IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798061469865,"sku":"2954T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2954t","provider":"Iright","version":"1.0","type":"link"}