{"product_id":"cst-2980s","title":"CST,  2980S, Caldesmon-1 Antibody","description":"Polyclonal Antibody for studying Caldesmon. Validated for Western Blotting. Highly specific and rigorously validated in-house, Caldesmon-1 Antibody (CST #2980) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCaldesmon-1 Antibody detects endogenous levels to total caldesmon-1 protein. Based on sequence homology, this antibody is expected to cross-react with both the smooth muscle and nonmuscle isoforms.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human caldesmon-1. Antibodies are purified using peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCaldesmon-1 is an actin filament stabilizing protein involved in the regulation of cell contraction. Binding of caldesmon-1 to actin is weakened by phosphorylation and by calmodulin in the presence of calcium. Caldesmon-1 is encoded by a single gene, which is spliced to generate a widely distributed low molecular weight form and a smooth muscle specific high molecular weight form (1,2). Caldesmon-1 is phosphorylated by the cyclin dependent kinase cdc2 and Erk1\/2 MAP kinase, both of which prevent the activity of caldesmon-1 (3-5). Phosphorylation of caldesmon-1 by cdc2 is required for passage of cells through mitosis (6). Phosphorylation by Erk1\/2 is important in regulating smooth muscle contraction (7). Caldesmon-1 activity may play a role in the formation of podosomes, adhesion complexes associated with the secretion of matrix metalloproteases, invasion, and metastasis (reviewed in 5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCAD; CALD1; Caldesmon; caldesmon 1; CDM; H-CAD; HCAD; L-CAD; LCAD; MGC21352; NAG22; testis secretory sperm-binding protein Li 227n\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 70-80 non-muscle, 120-150 smooth muscle\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798063206569,"sku":"2980S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2980s","provider":"Iright","version":"1.0","type":"link"}