{"product_id":"cst-2996t","title":"CST,  2996T, SET8 (C18B7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SET8. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, SET8 (C18B7) Rabbit Monoclonal Antibody (CST #2996) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSET8 (C18B7) Rabbit Monoclonal Antibody detects endogenous levels of total SET8 protein (both isoforms). This antibody also crossreacts with an unidentified protein at 50kDa.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to human SET8 protein. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nSET domain-containing lysine methyltransferase 8 (SET8), also known as PR\/SET domain-containing protein 7 (PR\/SET7), is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1-3). SET8 is a single-subunit enzyme that mono-methylates histone H4 on Lys20, preferably on nucleosomal substrates (1-3). SET8 protein levels and Histone H4 Lys20 methylation are cell cycle regulated, both increasing in S phase and peaking at G2\/M phase (4,5). SET8 interacts with the PCNA protein, associates with sites of active DNA synthesis, and is required for DNA replication and genome stability during S phase (5-7). Inhibition of SET8 using shRNA or siRNA results in arrest of replication forks, induction of double-stranded DNA breaks, and a Chk1-mediated cell-cycle arrest in S and G2\/M phases of the cell cycle (6,7). Furthermore, SET8 methylates p53 on Lys382, down regulating the pro-apoptotic and checkpoint activation functions of p53 (8). In response to DNA damage, SET8 expression levels decrease, allowing p53 to activate checkpoints and\/or apoptosis (8). Both the methylation of histone H4 Lys20 and p53 appear to be important for the functions of SET8 in S phase.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nH4-K20-HMTase KMT5A; H4-K20-HMTase SETD8; H4-K20-specific histone methyltransferase; H4K20-specific histone methyltransferase splice variant Set8b; Histone-lysine N-methyltransferase KMT5A; Histone-lysine N-methyltransferase SETD8; KMT5A; lysine (K)-specific methyltransferase 5A; lysine methyltransferase 5A; Lysine N-methyltransferase 5A; Lysine-specific methylase 5A; N-lysine methyltransferase KMT5A; N-lysine methyltransferase SETD8; PR-Set7; PR\/SET domain containing protein 8; PR\/SET domain-containing protein 07; PR\/SET07; PRSET7; SET domain containing (lysine methyltransferase) 8; SET domain-containing protein 8; SET07; SET8; SETD8\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 43\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798063927465,"sku":"2996T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-2996t","provider":"Iright","version":"1.0","type":"link"}