{"product_id":"cst-3014s","title":"CST,  3014S, Insulin (C27C9) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying insulin. Validated for WB,IHC,IF,IF,F. Available in 2 sizes. Highly specific and rigorously validated in-house, Insulin (C27C9) Rabbit Monoclonal Antibody (CST #3014) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nSimple Western™: 1:10 - 1:50\nImmunohistochemistry (Paraffin): 1:6400 - 1:25600\nImmunofluorescence (Frozen): 1:200 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\nFlow Cytometry (Fixed\/Permeabilized): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 56823 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nInsulin (C27C9) Rabbit Monoclonal Antibody detects endogenous levels of total insulin protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to Val42 of human insulin.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nIDDM; IDDM1; IDDM2; ILPR; ins; Insulin; insulin (A chain, B chain); Insulin A chain; Insulin B chain; IRDN; MODY10; PNDM4; preproinsulin; proinsulin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800250831017,"sku":"3014S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3014s","provider":"Iright","version":"1.0","type":"link"}