{"product_id":"cst-30177s","title":"CST,  30177S, G4S Linker (E7O2V) F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)","description":"Monoclonal Antibody for studying . Validated for Flow Cytometry (Live). Highly specific and rigorously validated in-house, G4S Linker (E7O2V) F(ab')2 Fragment (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 488 Conjugate) (CST #30177) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nFlow Cytometry (Live): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Flow Cytometry (Live)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nG4S Linker (E7O2V) F(ab')2 Fragment (Alexa Fluor\nSpecies Reactivity: Human, All Species Expected\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S) or 20-mer (G4S) within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2). Human F receptors exhibit strong cross-reactivity with rabbit IgG, and are expressed at high levels on cells of myeloid lineage (e.g., monocytes and myeloid-derived cell lines). Fluorescent anti-linker F(ab') fragment conjugates are ideal for use in flow cytometry assays on cells containing F receptors, and eliminate the need for an F blocking step prior to antibody incubation. Cell Signaling Technology employs strict quality control procedures to ensure that each conjugate provides optimal specificity and fluorescence.\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H All\nSENSITIVITY: Transfected Only\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800375087273,"sku":"30177S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-30177s","provider":"Iright","version":"1.0","type":"link"}