{"product_id":"cst-30239s","title":"CST,  30239S, Nicastrin (D4F6N) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Nicastrin. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Nicastrin (D4F6N) Rabbit Monoclonal Antibody (CST #30239) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\nImmunofluorescence (Frozen): 1:200\nImmunofluorescence (Immunocytochemistry): 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 35956 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nNicastrin (D4F6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total Nicastrin protein.\nSpecies Reactivity: Human, Mouse, Rat, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human Nicastrin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nNicastrin is a transmembrane glycoprotein serving as an essential component of the γ-secretase complex (1,2). Nicastrin is physically associated with presenilin and plays an important role in the stabilization and correct localization of presenilin to the membrane-bound γ-secretase complex (3). Nicastrin also serves as a docking site for γ-secretase substrates such as APP and Notch, directly binding to them and properly presenting them to γ-secretase to ensure the correct cleavage process (2,4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nanterior pharynx-defective 2; APH2; ATAG1874; KIAA0253; NCSTN; NICA; Nicastrin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R Mk\nSENSITIVITY: Endogenous\nMW (kDa): 110, 120\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798065598633,"sku":"30239S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-30239s","provider":"Iright","version":"1.0","type":"link"}