{"product_id":"cst-3026s","title":"CST,  3026S, Phospho-Insulin Receptor beta (Tyr1345) (14A4) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying InsR (Tyr1355) phosphate. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Insulin Receptor beta (Tyr1345) (14A4) Rabbit Monoclonal Antibody (CST #3026) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Insulin Receptor beta (Tyr1345) (14A4) Rabbit Monoclonal Antibody detects transfected levels of insulin receptor β only when phosphorylated at Tyr1345. This antibody slightly cross-reacts with activated IGF-I receptors and some other activated tyrosine kinases.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1345 of human insulin receptor β.\n\u003cb\u003eBackground\u003c\/b\u003e\nType I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146\/1150\/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCD220; HHF5; INSR; Insulin receptor; Insulin receptor subunit alpha; Insulin receptor subunit beta; IR\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Transfected Only\nMW (kDa): 95\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798065860777,"sku":"3026S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3026s","provider":"Iright","version":"1.0","type":"link"}