{"product_id":"cst-30325t","title":"CST,  30325T, F4\/80 (D4C8V) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying EMR1 mouse. Validated for WB,IP,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, F4\/80 (D4C8V) Rabbit Monoclonal Antibody (CST #30325) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\nImmunofluorescence (Frozen): 1:200 - 1:800\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:800\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 90302 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nF4\/80 (D4C8V) Rabbit Monoclonal Antibody recognizes endogenous levels of total F4\/80 protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln260 of mouse F4\/80 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nF4\/80 (EMR1) is a heavily glycosylated G-protein-coupled receptor and is a well-established marker for mouse macrophages (1-3). Expression of F4\/80 has also been observed in microglia and subset populations of dendritic cells (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAdgre1; Adhesion G protein-coupled receptor E1; AGRE1; Cell surface glycoprotein F4\/80; DD7A5-; DD7A5-7; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EGF-TM7; Emr; Emr1; EMR1 hormone receptor; F4\/80; Gpf480; Ly7; Ly71; lymphocyte antigen 71; TM7L; TM7LN3\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 65-250\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800087449769,"sku":"30325T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-30325t","provider":"Iright","version":"1.0","type":"link"}