{"product_id":"cst-30483t","title":"CST,  30483T, CDK6 (E3E3Q) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Cdk6. Validated for WB,WB,IP,IHC. Available in 2 sizes. Highly specific and rigorously validated in-house, CDK6 (E3E3Q) Rabbit Monoclonal Antibody (CST #30483) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:50 - 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 41769 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCDK6 (E3E3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total CDK6 protein.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CDK6 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1\/S phase restriction point (1). CDK6\/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1\/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCDK6; CDKN6; Cell division protein kinase 6; cyclin dependent kinase 6; Cyclin-dependent kinase 6; MCPH12; MGC59692; PLSTIRE; Serine\/threonine-protein kinase PLSTIRE\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 36\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798067105961,"sku":"30483T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-30483t","provider":"Iright","version":"1.0","type":"link"}