{"product_id":"cst-30625s","title":"CST,  30625S, SMCHD1 (F4G6Y) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying SMCHD1. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SMCHD1 (F4G6Y) Rabbit Monoclonal Antibody (CST #30625) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nSMCHD1 (F4G6Y) Rabbit Monoclonal Antibody recognizes endogenous levels of total SMCHD1 protein. This antibody is expected to detect only isoform 1 of SMCHD1.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human SMCHD1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nStructural maintenance of chromosomes flexible hinge domain-containing protein 1 (SMCHD1) is an epigenetic regulator and a noncanonical member of the structural maintenance of chromosomes (SMC) family, containing an N-terminal ATPase domain and a C-terminal hinge domain (1). This protein is critical for both X chromosome inactivation and silencing of clustered autosomal loci (1-5). Heterozygous mutations in are found in two distinct human disorders, facioscapulohumeral muscular dystrophy (FSHD) and the rare craniofacial disorder, Bosma arhinia and microphthalmia syndrome (BAMS) (6-8). More recently, it has been shown that SMCHD1 is required for long-range chromatin interactions on the inactive X chromosome and at its autosomal targets, including the imprinted loci (9-11). Loss of in cells results in altered chromatin modifications, DNA hypomethylation, and subsequent perturbations in gene expression. In the absence of SMCHD1, other epigenetic regulators such as CCCTC-binding factor (CTCF) are enriched at SMCHD1 targets (5,10,11). Importantly, various post-translational modifications such as phosphorylation, ubiquitination, and SUMOylation on SMCHD1 have been identified (12-14).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nBAMS; DKFZp686O0631; FSHD2; KIAA0650; SMC hinge domain-containing protein 1; SMCHD1; SMHD1; structural maintenance of chromosomes flexible hinge domain containing 1; Structural maintenance of chromosomes flexible hinge domain-containing protein 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 220\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798068056233,"sku":"30625S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-30625s","provider":"Iright","version":"1.0","type":"link"}