{"product_id":"cst-3070s","title":"CST,  3070S, Phospho-IRS-1 (Tyr895) Antibody","description":"Polyclonal Antibody for studying IRS-1 (Tyr896) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-IRS-1 (Tyr895) Antibody (CST #3070) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-IRS-1 (Tyr895) Antibody detects transfected levels of IRS-1 only when phosphorylated at Tyr895. This antibody may cross-react with other activated receptor tyrosine kinases (RTKs) and docking proteins.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr896 of human IRS-1. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nInsulin receptor substrate 1 (IRS-1) is one of the major substrates of the insulin receptor kinase (1). IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2-domain containing proteins that mediate the metabolic and growth-promoting functions of insulin (2-4). IRS-1 also contains over 30 potential serine\/threonine phosphorylation sites. Ser307 of IRS-1 is phosphorylated by JNK (5) and IKK (6) while Ser789 is phosphorylated by SIK-2, a member of the AMPK family (7). The PKC and mTOR pathways mediate phosphorylation of IRS-1 at Ser612 and Ser636\/639, respectively (8,9). Phosphorylation of IRS-1 at Ser1101 is mediated by PKCθ and results in an inhibition of insulin signaling in the cell, suggesting a potential mechanism for insulin resistance in some models of obesity (10). Phosphorylation of Tyr895 in IRS-1 provides a binding site for Grb2, which mediates the downstream signaling leading to MAP kinase activation and mitogenesis (11).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nHIRS-1; Insulin receptor substrate 1; IRS-1; IRS1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Transfected Only\nMW (kDa): 180\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798068351145,"sku":"3070S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3070s","provider":"Iright","version":"1.0","type":"link"}