{"product_id":"cst-3079t","title":"CST,  3079T, Phospho-Aurora A (Thr288) (C39D8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Aurora A (Thr288) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Aurora A (Thr288) (C39D8) Rabbit Monoclonal Antibody (CST #3079) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunofluorescence (Immunocytochemistry): 1:1600\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 49049 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunofluorescence (Immunocytochemistry)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-Aurora A (Thr288) (C39D8) Rabbit Monoclonal Antibody detects endogenous levels of Aurora A only when phosphorylated at Thr288.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr288 of human Aurora A.\n\u003cb\u003eBackground\u003c\/b\u003e\nAurora A (AIK) is a cell cycle-regulated Ser\/Thr protein kinase that is overexpressed in many tumor cell lines (1-3). Phosphorylation of Aurora A at Thr288 within the kinase activation loop results in a significant increase in its activity and may target the protein for proteasomal degradation during mitosis (4). The closely-related kinase Aurora B (AIM1) has been implicated in multiple mitotic events (5), and siRNA silencing of Aurora B expression results in reduced histone H3 phosphorylation, aberrant chromosome alignment\/segregation, and altered survivin localization (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAIK; AIRK1; ARK-1; ARK1; AURA; AURKA; Aurora 2; Aurora A; Aurora kinase A; Aurora-related kinase 1; aurora\/IPL1-like kinase; Aurora\/IPL1-related kinase 1; AURORA2; AYK1; Breast tumor-amplified kinase; breast-tumor-amplified kinase; BTAK; hARK1; IAK1; IPL1-related kinase; MGC34538; PPP1R47; protein phosphatase 1, regulatory subunit 47; serine\/threonine kinase 6; serine\/threonine protein kinase 15; Serine\/threonine-protein kinase 15; Serine\/threonine-protein kinase 6; Serine\/threonine-protein kinase aurora-A; STK15; STK6; STK7\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 48\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798068973737,"sku":"3079T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3079t","provider":"Iright","version":"1.0","type":"link"}