{"product_id":"cst-31169sf","title":"CST,  31169SF, p47phox (F3Y6C) Rabbit Monoclonal Antibody (BSA and Azide Free)","description":"Monoclonal Antibody for studying p47phox. Validated for WB,IHC,IHC,IF. Highly specific and rigorously validated in-house, p47phox (F3Y6C) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #31169) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nThis product is the carrier free version of product #63290. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT\u0026amp;RUN or CUT\u0026amp;Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions\/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.\n\u003cb\u003eFormulation\u003c\/b\u003e\nSupplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 63290\n\u003cb\u003eStorage\u003c\/b\u003e\nStore at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze\/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\np47phox (F3Y6C) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total p47phox protein. This antibody is not approved for immunofluorescence on frozen mouse tissue due to low sensitivity. This antibody detects a 30 kDa band of unknown origin in some samples.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala227 of human p47phox protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe phagocytic NADPH oxidase is a multiprotein enzyme that catalyzes the reduction of oxygen to superoxide in response to pathogenic invasion. The NADPH oxidase consists of 6 subunits, including the membrane-bound p91 phox and p22 phox heterodimers (also known as cytochrome b558), the cytosolic complex of p40phox, p47phox and p67phox, and the small GTPase Rac2. Activation of NADPH oxidase is initiated by cytosolic complex phosphorylation, which induces a conformational change that leads to the translocation of the cytosolic complex to the membrane and formation of an active enzyme with cytochrome b558 (1). Defects in p47phox, often resulting from recombination between p47phox and a nearby homologous pseudogene, cause chronic granulomatous disease (2-4). Elevated oxidative stress due to increased myocardial NADPH oxidase activity may be a contributing factor in heart failure (5,6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n47 kDa autosomal chronic granulomatous disease protein; 47 kDa neutrophil oxidase factor; CGD1; FLJ79451; NADPH oxidase organizer 2; NCF-1; NCF-47K; NCF1; NCF1A; Neutrophil cytosol factor 1; neutrophil cytosolic factor 1; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1); Neutrophil NADPH oxidase factor 1; Nox organizer 2; Nox-organizing protein 2; NOXO2; p47-phox; p47phox; SH3 and PX domain-containing protein 1A; SH3PXD1A\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 47\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798071431337,"sku":"31169SF","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-31169sf","provider":"Iright","version":"1.0","type":"link"}