{"product_id":"cst-31219s","title":"CST,  31219S, COX2\/MT-CO2 Antibody","description":"Polyclonal Antibody for studying COX2\/MT-CO2. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, COX2\/MT-CO2 Antibody (CST #31219) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nCOX2\/MT-CO2 Antibody recognizes endogenous levels of total COX2\/MT-CO2 protein. This antibody does not cross-react with COX1\/MT-CO1 or COX3\/MT-CO3.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro223 of human COX2\/MT-CO2 protein. Antibodies are purified by peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe electron transport chain (or respiratory chain) is a series of mitochondrial protein complexes (Complexes I-IV) that utilize coupled reduction-oxidation (redox) reactions to transfer electrons from donor molecules to acceptors. By coupling this electron transport with active proton pumping across the inner mitochondrial membrane, an electrochemical gradient is generated that is used to drive ATP synthesis (1). Complex IV (cytochrome c oxidase) is the terminal complex of the respiratory chain, responsible for transporting electrons from cytochrome c to molecular oxygen. COX2 (cytochrome c oxidase subunit 2), also known as MT-CO2, is one of 14 subunits of cytochrome c oxidase, and one of three subunits (COX1\/MT-CO1, COX2\/MT-CO2, and COX3\/MT-CO3) encoded by the mitochondrial genome (2). These three mitochondrial-encoded subunits form the functional core of cytochrome c oxidase. Research studies have shown that disruptions to COX2\/MT-CO2 function can have deleterious health effects. For example, a novel point mutation in the gene was identified as a risk factor for cardiovascular disease (3), whereas a separate point mutation was causally linked to adult onset cerebellar ataxia (4).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nCOII; COX2; COXII; Cytochrome c oxidase polypeptide II; Cytochrome c oxidase subunit 2; cytochrome c oxidase subunit II; mitochondrially encoded cytochrome c oxidase II; MT-CO2; MTCO2\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 19\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46798071791785,"sku":"31219S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-31219s","provider":"Iright","version":"1.0","type":"link"}