{"product_id":"cst-31474s","title":"CST,  31474S, Pellino-1 (D2Z4F) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PELI1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Pellino-1 (D2Z4F) Rabbit Monoclonal Antibody (CST #31474) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPellino-1 (D2Z4F) Rabbit Monoclonal Antibody recognizes endogenous levels of total Pellino-1 protein.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala403 of human Pellino-1 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nPellino-1 (PELI1) is an E3 ubiquitin ligase that regulates activation of various immune pathways in different contexts and cell types (1). Pellino-1 controls NFκB activation downstream of the interleukin-1 receptor and TLRs through association with and ubiquitination of IRAK1 (1-4). Pellino-1 was also demonstrated to regulate the NFκB pathway in T cells by targeting c-Rel for degradation, thereby enabling suppression by regulatory T cells, maintaining peripheral tolerance, and preventing autoimmunity in mice (5). Finally, in experimental autoimmune encephalomyelitis (EAE), the mouse model of multiple sclerosis, Pellino-1 contributes to inflammation by promoting degradation of Traf3 leading to induction of chemokines and proinflammatory cytokines (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nDKFZp686C18116; E3 ubiquitin-protein ligase pellino homolog 1; MGC50990; PELI1; pellino E3 ubiquitin protein ligase 1; pellino homolog 1; pellino homolog 1 (Drosophila); Pellino-1; Pellino-related intracellular-signaling molecule; PRISM; Protein pellino homolog 1; RING-type E3 ubiquitin transferase pellino homolog 1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 47\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798073725097,"sku":"31474S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-31474s","provider":"Iright","version":"1.0","type":"link"}