{"product_id":"cst-3153s","title":"CST,  3153S, Progesterone Receptor A\/B (C89F7) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Progesterone receptor. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, Progesterone Receptor A\/B (C89F7) Rabbit Monoclonal Antibody (CST #3153) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 59153 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nProgesterone Receptor A\/B (C89F7) Rabbit Monoclonal Antibody detects endogenous levels of total progesterone receptor A and B proteins. This antibody does not cross-react with other PR family members. Non-specific staining of smooth muscle may be observed in paraffin-embedded tissues.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr541 of human progesterone receptor.\n\u003cb\u003eBackground\u003c\/b\u003e\nHuman progesterone receptor (PR) is expressed as two forms: the full length PR-B and the short form PR-A. PR-A lacks the first 164 amino acid residues of PR-B (1,2). Both PR-A and PR-B are ligand activated, but differ in their relative ability to activate target gene transcription (3,4). The activity of PR is regulated by phosphorylation; at least seven serine residues are phosphorylated in its amino-terminal domain. Three sites (Ser81, Ser102, and Ser162) are unique to full length PR-B, while other sites (Ser190, Ser294, Ser345, and Ser400) are shared by both isoforms (5). Phosphorylation of PR-B at Ser190 (equivalent to Ser26 of PR-A) is catalyzed by CDK2 (6). Mutation of Ser190 results in decreased activity of PR (7), suggesting that the phosphorylation at Ser190 may be critical to its biological function.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nNR3C3; Nuclear receptor subfamily 3 group C member 3; PGR; PR; PRGR; Progesterone receptor\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 90 (PR-A) and 118 (PR-B)\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46798074052777,"sku":"3153S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3153s","provider":"Iright","version":"1.0","type":"link"}