{"product_id":"cst-31659l","title":"CST,  31659L, cGAS (D3O8O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying cGAS mouse. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 3 sizes. Highly specific and rigorously validated in-house, cGAS (D3O8O) Rabbit Monoclonal Antibody (CST #31659) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:50 - 1:250\nImmunoprecipitation: 1:200\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\ncGAS (D3O8O) Rabbit Monoclonal Antibody recognizes endogenous levels of mouse cGAS protein.\nSpecies Reactivity: Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala66 of mouse cGAS protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCyclic GMP-AMP synthase (cGAS, MB21D1) is an antiviral enzyme that produces the second messenger cyclic-GMP-AMP (cGAMP) in response to cytoplasmic DNA (1,2). The cGAS protein acts as a cytosolic DNA sensor that binds DNA and produces the cGAMP second messenger from ATP and GTP (1,2). cGAMP binds to and activates STING, a transmembrane adaptor protein that is a critical component of the cellular innate immune response to pathogenic cytoplasmic DNA (1-4). STING is ubiquitously expressed and found predominantly in the ER (3). Following activation, STING translocates with TBK1 to perinuclear endosomes (5). The TBK1 kinase phosphorylates and activates interferon regulatory factors (IRFs) and NF-κB, which leads to the induction of type I interferon and other immune response genes (3-5).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n2'3'-cGAMP synthase; cGAMP synthase; cGAS; Cyclic GMP-AMP synthase; E330016A19Rik; FLJ39829; hypothetical protein LOC214763; m-cGAS; Mab-21 domain containing 1; Mab-21 domain-containing protein 1; Mb21d1; OTTMUSP00000021298; RIKEN cDNA E330016A19 gene\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M\nSENSITIVITY: Endogenous\nMW (kDa): 62\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800088170665,"sku":"31659L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-31659l","provider":"Iright","version":"1.0","type":"link"}