{"product_id":"cst-3179l","title":"CST,  3179L, Phospho-PERK (Thr980) (16F8) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying PERK (Thr980) phosphate mouse. Validated for Western Blotting. Available in 3 sizes. Highly specific and rigorously validated in-house, Phospho-PERK (Thr980) (16F8) Rabbit Monoclonal Antibody (CST #3179) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-PERK (Thr980) (16F8) Rabbit Monoclonal Antibody detects endogenous levels of PERK phosphorylated at Thr980.\nSpecies Reactivity: Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr980 of mouse PERK.\n\u003cb\u003eBackground\u003c\/b\u003e\nProtein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nAI427929; E2AK3; Eif2ak3; eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; Pancreatic eIF2-alpha kinase; Pek; Perk; PRKR-like endoplasmic reticulum kinase\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: M R\nSENSITIVITY: Endogenous\nMW (kDa): 170\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800088563881,"sku":"3179L","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3179l","provider":"Iright","version":"1.0","type":"link"}