{"product_id":"cst-31912s","title":"CST,  31912S, Phospho-RPA32\/RPA2 (Ser8) (E5A2F) Rabbit Monoclonal Antibody (Alexa Fluor® 488 Conjugate)","description":"Monoclonal Antibody for studying RPA32 (Ser8) phosphate. Validated for Immunofluorescence (Immunocytochemistry),Flow Cytometry (Fixed\/Permeabilized). Highly specific and rigorously validated in-house, Phospho-RPA32\/RPA2 (Ser8) (E5A2F) Rabbit Monoclonal Antibody (Alexa Fluor\u003csup\u003e®\u003c\/sup\u003e 488 Conjugate) (CST #31912) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nImmunofluorescence (Immunocytochemistry): 1:200 - 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg\/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-RPA32\/RPA2 (Ser8) (E5A2F) Rabbit Monoclonal Antibody (Alexa Fluor\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser8 of human RPA32\/RPA2 protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nRPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32\/30, and 14 kDa subunits, collectively known as RPA. RPA is a single-stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch, and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9\/Rad1\/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Phosphorylation of RPA32 occurs at serines 4, 8, and 33 (11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA, and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nREPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 34 kDa subunit; replication protein A2; replication protein A2, 32kDa; RF-A protein 2; RFA2; RP-A p32; RP-A p34; RPA2; RPA32; RPA34\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800377544873,"sku":"31912S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-31912s","provider":"Iright","version":"1.0","type":"link"}