{"product_id":"cst-3195t","title":"CST,  3195T, E-Cadherin (24E10) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying E-Cadherin. Validated for WB,WB,IHC,IHC,IF,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, E-Cadherin (24E10) Rabbit Monoclonal Antibody (CST #3195) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:10 - 1:50\nIHC Leica Bond: 1:400 - 1:1600\nImmunohistochemistry (Paraffin): 1:400\nImmunofluorescence (Frozen): 1:1600\nImmunofluorescence (Immunocytochemistry): 1:1600\nFlow Cytometry (Fixed\/Permeabilized): 1:100 - 1:400\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier-free (BSA and Azide Free) version of this product see product # 96743 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, IHC Leica Bond, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nE-Cadherin (24E10) Rabbit Monoclonal Antibody detects endogenous levels of total E-cadherin protein. This antibody does not cross-react with related family members, such as N-cadherin.\nSpecies Reactivity: Human, Mouse\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nCadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers (1-3). Research studies indicate that cancer cells have upregulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the \"cadherin switch.\" N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion (3). Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nArc-1; BCDS1; CADH1; Cadherin; cadherin 1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1; cadherin 1, type 1, E-cadherin (epithelial); Cadherin E; Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120\/80; CD324; CDH1; CDHE; cell-CAM 120\/80; E-Cad\/CTF1; E-Cad\/CTF2; E-Cad\/CTF3; E-cadherin; E-cadherin 1; ECAD; epididymis secretory sperm binding protein; Epithelial cadherin; LCAM; UVO; Uvomorulin\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M\nSENSITIVITY: Endogenous\nMW (kDa): 135\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800970416297,"sku":"3195T","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3195t","provider":"Iright","version":"1.0","type":"link"}