{"product_id":"cst-3197s","title":"CST,  3197S, EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying EGFR (Leu858Arg) mutation. Validated for WB,WB,IP,IHC,IF,F. Available in 3 sizes. Highly specific and rigorously validated in-house, EGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody (CST #3197) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nSimple Western™: 1:250\nImmunoprecipitation: 1:200\nImmunohistochemistry (Paraffin): 1:100\nImmunofluorescence (Immunocytochemistry): 1:400\nFlow Cytometry (Fixed\/Permeabilized): 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 28110 .\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed\/Permeabilized)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nEGF Receptor (L858R Mutant Specific) (43B2) Rabbit Monoclonal Antibody detects endogenous levels of EGFR mutant L858R protein. This antibody may cross-react with wildtype EGFR and other HER family members when highly over-expressed. Careful titration of this antibody may be required to obtain optimal specificity.\nSpecies Reactivity: Human\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to L858R mutant sequence of human EGF receptor.\n\u003cb\u003eBackground\u003c\/b\u003e\nThe epidermal growth factor (EGF) receptor is a 170 kDa transmembrane tyrosine kinase that belongs to the HER\/ErbB protein family. Research studies have shown that somatic mutations in the tyrosine kinase domain of EGF receptor (EGFR) are present in a subset of lung adenocarcinomas that respond to EGFR inhibitors, such as gefitinib and erlotinib (1-3). Two types of mutations account for approximately 90% of mutated cases: a specific point mutation, L858R, that occurs in exon 21 and short in-frame deletions in exon 19 (4,5). The most frequent exon 19 deletion is E746-A750, accounting for 90% of lesions at this site, although some rare variants occur.\n\u003cb\u003eAlternate Names\u003c\/b\u003e\navian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EGF receptor; EGFR; Epidermal growth factor receptor; epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian); epidermal growth factor receptor tyrosine kinase domain; erb-b2 receptor tyrosine kinase 1; ERBB; ERBB1; HER1; mENA; NISBD2; PIG61; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H\nSENSITIVITY: Endogenous\nMW (kDa): 175\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800970645673,"sku":"3197S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3197s","provider":"Iright","version":"1.0","type":"link"}