{"product_id":"cst-32248s","title":"CST,  32248S, Phospho-beta-Dystroglycan (Tyr892) (E6T9O) Rabbit Monoclonal Antibody","description":"Monoclonal Antibody for studying Dystroglycan (Tyr892) phosphate. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-beta-Dystroglycan (Tyr892) (E6T9O) Rabbit Monoclonal Antibody (CST #32248) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunoprecipitation: 1:100\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunoprecipitation\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nPhospho-beta-Dystroglycan (Tyr892) (E6T9O) Rabbit Monoclonal Antibody recognizes endogenous levels of β-dystroglycan protein only when phosphorylated at Tyr892. By western blot, this antibody detects bands of unknown origin at approximately 80 and 140 kDa.\nSpecies Reactivity: Human, Mouse, Rat\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nMonoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr892 of human β-dystroglycan protein.\n\u003cb\u003eBackground\u003c\/b\u003e\nDystroglycan 1 (DAG1) is a ubiquitously expressed glycoprotein that is post-translationally processed into two protein subunits. α-dystroglycan is secreted and localizes to the outer plasma membrane, where it binds to the transmembrane subunit, β-dystroglycan. The two proteins form a complex that connects the cytoskeleton with the extracellular matrix and plays essential roles in various cellular processes, including neuromuscular junction function and adhesion-driven cell signaling (1,2). Phosphorylation of β-dystroglycan at Tyr892 regulates protein-protein interaction and subcellular localization (3-5). β-dystroglycan interacts with nuclear envelope proteins emerin and lamin B1 to regulate nuclear architecture and function (6).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\n156DAG; A3a; AGRNR; Alpha-DG; Alpha-dystroglycan; Beta-DG; Beta-dystroglycan; DAG; DAG1; Dystroglycan; Dystroglycan 1; dystroglycan 1 (dystrophin-associated glycoprotein 1); Dystrophin-associated glycoprotein 1; LGMDR16; MDDGA9; MDDGC7; MDDGC9\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H M R\nSENSITIVITY: Endogenous\nMW (kDa): 45\nSource\/Isotype: Rabbit IgG","brand":"CST","offers":[{"title":"Default Title","offer_id":46800972972201,"sku":"32248S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-32248s","provider":"Iright","version":"1.0","type":"link"}