{"product_id":"cst-3227s","title":"CST,  3227S, G-beta-L Antibody","description":"Polyclonal Antibody for studying GbetaL. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, G-beta-L Antibody (CST #3227) is ready to ship.\n\n\u003cb\u003eProduct Usage Information\u003c\/b\u003e\nWestern Blotting: 1:1000\nImmunohistochemistry (Paraffin): 1:50\n\u003cb\u003eStorage\u003c\/b\u003e\nSupplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg\/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody.\n\u003cb\u003eProtocol\u003c\/b\u003e\nAvailable protocols: Western Blotting, Immunohistochemistry (Paraffin)\n\u003cb\u003eSpecificity \/ Sensitivity\u003c\/b\u003e\nG-beta-L Antibody detects endogenous levels of total GβL protein.\nSpecies Reactivity: Human, Monkey\n\u003cb\u003eSource \/ Purification\u003c\/b\u003e\nPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln164 of human GβL. Antibodies are purified by protein A and peptide affinity chromatography.\n\u003cb\u003eBackground\u003c\/b\u003e\nCell growth is a fundamental biological process whereby cells accumulate mass and increase in size. The mammalian Target of Rapamycin (mTOR) pathway regulates growth by coordinating energy and nutrient signals with growth factor-derived signals (1). mTOR is a large protein kinase with two different complexes. One complex contains mTOR, GβL, and raptor, which is a target of rapamycin. The other complex, insensitive to rapamycin, includes mTOR, GβL, and rictor (1). GβL associates with the kinase domain of mTOR and stimulates mTOR kinase activity (2). A reduction in GβL expression has been shown to decrease phosphorylation of S6K1 (2).\n\u003cb\u003eAlternate Names\u003c\/b\u003e\nG protein beta subunit like; G protein beta subunit-like; Gable; GbetaL; GBL; LST8; Mammalian lethal with SEC13 protein 8; MGC111011; MLST8; MTOR associated protein, LST8 homolog; MTOR associated protein, LST8 homolog (S. cerevisiae); POP3; Protein GbetaL; Target of rapamycin complex subunit LST8; TORC subunit LST8; WAT1\n\n\u003cb\u003eSpecification\u003c\/b\u003e\n\nREACTIVITY: H Mk\nSENSITIVITY: Endogenous\nMW (kDa): 37\nSOURCE: Rabbit","brand":"CST","offers":[{"title":"Default Title","offer_id":46800973201577,"sku":"3227S","price":0.99,"currency_code":"USD","in_stock":true}],"url":"https:\/\/iright.com\/products\/cst-3227s","provider":"Iright","version":"1.0","type":"link"}